Fig. 4
From: Simplified plasmid cloning with a universal MCS design and bacterial in vivo assembly
Effects of the linearization method and homologous length on assembly efficiency. a Transformation efficiency (shown as CFU/plate and positive ratio of colonies) of PCR-linearized pUC19-UMCS-S-EGFP with the mCherry sequence flanked by 6-, 9-, 12-, 15-, and 18-bp homologous linkers (n = 4). b and c Transformation efficiency of SalI- and EcoRV-digested pUC19-UMCS-S-EGFP and pUC19-UMCS-E-EGFP with the mCherry sequence flanked by 9-, 12-, 15-, and 18-bp homologous linkers (n = 4)