Table 1 Primers used in the study (from IDT or Sigma). gRNA target sequence underlined
From: Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system
| Primers name | Primer sequence | Annealing temp (°C) |
|---|---|---|
| EGFP_Fw_gRNA | caccgggcgaggagctgttcaccg | N/A |
| EGFP_Rv_gRNA | aaaccggtgaacagctcctcgccc | N/A |
| EGFP_Fw_seq (CMV-F) | cgcaaatgggcggtaggcgtg | 63.5 |
| EGFP_Rv_seq | gctgaagcactgcacgccgt | |
| U6_Fw_Seq (hU6-F) | gagggcctatttcccatgatt | N/A |
| Otsha_RIG-I_Fw_gRNA | caccgcgttccagaggttgcagag | N/A |
| Otsha_RIG-I_Rv_gRNA | aaacctctgcaacctctggaacgc | N/A |
| Otsha_RIG-I_Fw_seq | aagtgtcaaagtacagcaattctagc | 64 |
| Otsha_RIG-I_Rv_seq | cttccagggcatctagcagg | |
| Otsha_RIG-I_OffT1_Fw_seq Offfasasfafqfaf’fI_Off1_Fw_sec | tgttgatctctgccttgcac | 64 |
| Otsha_RIG-I_OffT1_Rv_seq | tatcccaccttggttgcttc | |
| Otsha_RIG-I_OffT2_Fw_sec | cggacaacaggccattagat | 64 |
| Otsha_RIG-I_OffT2_Rv_sec | tggcgtactgcactagcatc |