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| Gene | Native TIR (au) | Regulated TIR (au) | Native RBS (5′ – 3′) | Synthetic RBS (5′ – 3′) a |
|---|
| dsbA | 38,952 | 387,845 | //−tgtaTTAATCGGAGAGAGTAGATCatga−// | //−gatcAACTAAGGAGGCTTATTatga−// |
| dsbB | 158 | 1783 | //−tatgCATATTGCAGGGAAATGATTatgt−// | //−gattGCGCACACCGAAGTTGGAATACGATTGatgt−// |
| skp | 19,377 | 80,140 | //−ccggTGCAAATGGGATGGTAAGGAGTTTATTgtga−// | //−ccggCAGTATATACGTCTAACACTTAGGGGGAATATTAtga−// |
| sppA | 4205 | 19,838 | //−tattGCGCCTGTGACAGGTGTGACCTTAAGTTGGGAGAATACatgc−// | //−tattCAAACGTACCCCTTAATTATACCTAACGAGGAGAAACTatgc−// |
| degP | 2139 | 286 | //−ttttGCGTTATCTGTTAATCGAGACTGAAATACatga−// | //−ttttGAGTAACGCCACTGATCGAAATTGAGGAAGtga−// |
- a Desired TIR values for skp and degP were obtained by RBS replacement and substitution of the first base (underlined above) of a start codon; GTG → ATG and ATG → GTG, respectively
