Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection

Table 1 Primer table. Table with the primers and probes used in the qPCR assays

Target	Primers	Sequences	Amplicon (bp)	References
Actin = Plant reference gene	Actin	Forward 5′-CAAGCAGCATGAAGATCAAGGT-3’	103	[24]
		Reverse 5′-CACATCTGTTGGAAAGTGCTGAG-3’
		Probe 5′-HEX-CCTCCAATCCAGACACTGTACTTYCTCTC-BHQ-3’
CaMV Promoter 35S	P35S	Forward 5′-CGTCTACAAAGCAAGTGGATTG-3’	79	[25]
		Reverse 5′-TCTTGCGAAGGATAGTGGGATT-3’
		Probe 5′-FAM-TCTCCACTGACGTAAGGGATGACGCA-QSY-3′
CaMV gene P3	P3	Forward 5′-TGAAATCCTCAGTGACCAAAAATC-3′	152	[23]
		Reverse 5′-TACAAGGACAATCATTGATGAGC-3′
		Probe 5′-ABY-AAGCCGTTGCAGCGAAAATCGTTAATGA-QSY-3’
A.tumefaciens nopaline synthase terminator	TNOS	Forward 5′-GTCTTGCGATGATTATCATATAATTTCTG-3’	151	[24]
		Reverse 5′-CGCTATATTTTGTTTTCTATCGCGT-3’
		Probe 5′-JUN-AGATGGGTTTTTATGATTAGAGTCCCGCAA-QSY-3′

ISSN: 1472-6750